In 2014, Locklear featured in a public service announcement titled "We Are, Sarah Jones" in memory of camera assistant Sarah Jones who was killed in an accident on the set of Midnight Rider on February 20, 2014.
In 2012, Locklear began appearing in a recurring role on the TV Land comedy series Hot in Cleveland.
In 2013, she became a regular on the comedy-drama Franklin & Bash.
Throughout her career, Locklear has appeared in eight television productions made by Aaron Spelling, these being Dynasty, T. Hooker, Matt Houston, Fantasy Island, The Love Boat, Hotel, Melrose Place, and the made-for-TV movie Rich Men, Single Women.
Locklear was the guest host on Saturday Night Live on May 14, 1994.
Her other notable television roles include Sammy Jo Carrington on Dynasty (1981–89), Officer Stacy Sheridan on T. Hooker (1982–86), and Caitlin Moore on Spin City (1999–2002), for which she earned a further two Golden Globe nominations for Best Actress – Television Series Musical or Comedy.
She had a recurring role on the TV Land sitcom Hot in Cleveland and a main role on the TNT drama-comedy television series Franklin & Bash in 2013.Two of her best-known skits included her as an elderly Jewish woman on Coffee Talk with Mike Myers, and her acting as Amanda Woodward in a Wayne's World skit with Myers, Chris Farley, Adam Sandler, Rob Schneider, and Phil Hartman, where Wayne has a dream that he is a character on Melrose Place.Locklear also made a cameo in Wayne's World 2 in 1993.She was also the subject of a Lifetime Intimate Portrait special, and of an E! After Melrose Place ended in 1999, Locklear was cast in the sitcom Spin City (initially opposite Michael J. The series ended in 2002, but Locklear earned two Golden Globe Award nominations for Best Actress in a Television Series – Comedy.In 2002, Locklear had a brief recurring role on the sitcom Scrubs.In 2004, Locklear made a guest appearance on the sitcom Two and a Half Men, which starred her former Spin City co-star Charlie Sheen.